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Neuroscience

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JoVE Journal Neuroscience
Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
 

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

Article DOI: 10.3791/50487-v • 12:38 min • September 16th, 2013
September 16th, 2013 •
1,2, 1,2, 1,2
1Section of Endocrinology, Denver VA Medical Center, 2Department of Medicine, University of Colorado Denver School of Medicine

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Human neuroprogenitor cells (NPCs) were expanded under proliferating conditions. NPCs were differentiated into neuron-rich cultures in the presence of a combination of neurotrophins. Neuronal markers were detected by immunofluorescence staining. To isolate a pure population of neurons, NPCs were differentiated on PEN membrane slides and laser capture microdissection was performed.

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Laser Capture Microdissection Neurons Differentiated Human Neuroprogenitor Cells Culture NPCs Epidermal Growth Factor Fibroblast Growth Factor Nerve Growth Factor Brain-derived Neurotrophic Factor Dibutyryl CAMP Retinoic Acid Poly-L-lysine Mouse Laminin Astrocytes Ciliary Neurotrophic Factor Immunofluorescence Staining Neuronal Markers NeuN Synapsin Acetylcholinesterase Synaptophysin GAP43 Glial Fibrillary Acidic Protein STAT3 Polyethylene Naphthalate (PEN) Membrane Slides
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