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Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
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Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
Chapitres
- 00:00Titre
- 01:51Expansion and Differentiation of Human Neuroprogenitor Cells
- 05:28Immunofluorescence Staining of Differentiated Neuroprogenitor
- 10:17Results: Laser Capture Microdissection of Differrntiated Neurons
- 12:14Conclusion
Human neuroprogenitor cells (NPCs) were expanded under proliferating conditions. NPCs were differentiated into neuron-rich cultures in the presence of a combination of neurotrophins. Neuronal markers were detected by immunofluorescence staining. To isolate a pure population of neurons, NPCs were differentiated on PEN membrane slides and laser capture microdissection was performed.
