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骨髄由来マクロファージを用いたマクロファージ分極の調査
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
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骨髄由来マクロファージを用いたマクロファージ分極の調査

64,809 Views

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10:07 min

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June 23, 2013

DOI:

10.3791/50323-v

DOI:
10.3791/50323-v

•

10:07 min
June 23, 2013

•

68 Views
, , , ,
1Department of Animal Science,Texas A&M University, 2Department of Biology,Texas A&M University, 3Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University

Summary

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記事は容易に簡単に適応を説明<em> in vitroで</emマクロファージ分極を調査する>モデル。 GM-CSF/M-CSFの存在下で、骨髄からの造血幹/前駆細胞は、M1またはM2の刺激に続いて、単球分化に向けられる。活性化状態は、細胞表面抗原、遺伝子発現および細胞シグナル伝達経路の変化によって追跡することができる。

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Cite this Article

Ying, W., Cheruku, P. S., Bazer, F. W., Safe, S. H., Zhou, B. Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages. J. Vis. Exp. (76), e50323, doi:10.3791/50323 (2013).

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