Journal
/
Biologia
/
为外植体含量的颅间充质细胞行为评定
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
Please note that all translations are automatically generated. Click here for the English version.

为外植体含量的颅间充质细胞行为评定

DOI:
10.3791/4245-v

•

07:45 min

•

January 20, 2013

•

,
1Center for Neuroscience Research, Children’s Research Institute,Children’s National Medical Center

Capítulos

  • 00:05Título
  • 01:33Preparation and Set-up
  • 02:56Collection of Embryos
  • 04:38Preparation of Explants
  • 06:49Migration of Cranial Mesenchyme Cells from Explants
  • 07:21Conclusion

Summary

Tadução automática

Tadução automática

颅间充质经历了戏剧性的形态发生变动，可能提供的驱动力升高的神经褶<sup1,2</sup>。在这里，我们描述一个简单的<em>体外</em>外植体试验，在神经胚形成的颅间充质细胞的行为特征。此法有许多应用，包括适合药物处理和实时成像分析。

Tags

Explant AssayCellular BehaviorCranial MesenchymeCentral Nervous SystemNeural PlateNeurulationMorphogenetic MovementsNeural Tube FormationParaxial MesodermNeural Crest CellsPorous MeshworkStellate Shaped CellsExtracellular Matrix (ECM) StrandsStereotypical RearrangementsExpansionDriving ForcePathwaysCellular BehaviorsInteractions Between ECM And CellsEx Vivo AssayPharmacological ManipulationsSignaling PathwaysLive Imaging AnalysesMigratory Properties

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

微流体装置与Groove的模式，为研究细胞行为

Nanogenomedicine在MCF - 7细胞的细胞毒性：MTT法

为贴壁细胞培养的氧气插入的制备及操作

克隆形成含量：贴壁细胞

颅面间质细胞的电

细胞形态变化，从三维荧光图像量化的分析工具，

实时成像检测方法的评估钙的作用<sup> 2 +</sup>和鞘磷脂酶在成孔毒素伤口的修复

测量细菌的影响<em> C。线虫</em>行为使用一个鸡蛋保留含量

用于评估细胞运动和相关信号事件的机械控制的单细胞Durotaxis测定

使用 Vivo 法戈西西亚赛评估成人嗜血杆菌黑色粒细胞的年龄特异性噬细胞能力

Read Article