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An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
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Biología
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JoVE Revista Biología
An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

DOI:
10.3791/4245-v

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07:45 min

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January 20, 2013

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,
1Center for Neuroscience Research, Children’s Research Institute,Children’s National Medical Center

Capítulos

  • 00:05Título
  • 01:33Preparation and Set-up
  • 02:56Collection of Embryos
  • 04:38Preparation of Explants
  • 06:49Migration of Cranial Mesenchyme Cells from Explants
  • 07:21Conclusion

Summary

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Traducción Automática

The cranial mesenchyme undergoes dramatic morphogenic movements that likely provides a driving force for elevation of the neural folds1,2. Here we describe a simple ex vivo explant assay to characterize the cellular behaviors of the cranial mesenchyme during neurulation. This assay has numerous applications including being amenable to pharmacological manipulations and live imaging analyses.

Tags

Explant AssayCellular BehaviorCranial MesenchymeCentral Nervous SystemNeural PlateNeurulationMorphogenetic MovementsNeural Tube FormationParaxial MesodermNeural Crest CellsPorous MeshworkStellate Shaped CellsExtracellular Matrix (ECM) StrandsStereotypical RearrangementsExpansionDriving ForcePathwaysCellular BehaviorsInteractions Between ECM And CellsEx Vivo AssayPharmacological ManipulationsSignaling PathwaysLive Imaging AnalysesMigratory Properties

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