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高分辨率实时成像发展中的神经上皮细胞的行为
JoVE Journal
Neurociência
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JoVE Journal Neurociência
High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium
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高分辨率实时成像发展中的神经上皮细胞的行为

DOI:
10.3791/3920-v

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10:59 min

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April 12, 2012

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, , ,
1Neural Development Group, Division of Cell and Developmental Biology, College of Life Sciences,University of Dundee, Dundee, UK, 2Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences,University of Dundee, Dundee, UK

Capítulos

  • 00:05Título
  • 01:33Embryo Electroporation
  • 03:04Collagen and Slice Culture Medium Preparation
  • 04:22Slice Culture Preparation
  • 07:52Imaging Embryo Slices
  • 08:58Results: Time-lapse Sequences of Spinal Cord Progenitor Cells
  • 10:32Conclusion

Summary

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Tadução automática

实时成像胚胎组织挑战过很长一段时间。在这里，我们提出了一个小鸡脊髓细胞和亚细胞的变化，具有高时空分辨率的长期监测分析。这种技术可以适应其他地区的神经系统和胚胎发育。

Tags

High-resolutionLive ImagingCell BehaviorDeveloping NeuroepitheliumEmbryonic Spinal CordCycling Neural Progenitor CellsNeuronal DifferentiationMolecular MechanismsNeurogenesisReal-time ImagingCell ViabilityCulturePhototoxic EffectsFluorescent ImagingEx Vivo Slice Culture ProtocolWide-field Fluorescence MicroscopyTime-lapse MonitoringSpatial ResolutionTemporal ResolutionCellular BehaviorsSub-cellular BehaviorsGene Activity ReportersNotch Signaling

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