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High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

13,081 Views

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10:59 min

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April 12, 2012

DOI:

10.3791/3920-v

DOI:
10.3791/3920-v

•

10:59 min
April 12, 2012

•

4 Views
, , ,
1Neural Development Group, Division of Cell and Developmental Biology, College of Life Sciences,University of Dundee, Dundee, UK, 2Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences,University of Dundee, Dundee, UK

Summary

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Imaging embryonic tissue in real-time is challenging over long periods of time. Here we present an assay for monitoring cellular and sub-cellular changes in chick spinal cord for long periods with high spatial and temporal resolution. This technique can be adapted for other regions of the nervous system and developing embryo.

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High-resolutionLive ImagingCell BehaviorDeveloping NeuroepitheliumEmbryonic Spinal CordCycling Neural Progenitor CellsNeuronal DifferentiationMolecular MechanismsNeurogenesisReal-time ImagingCell ViabilityCulturePhototoxic EffectsFluorescent ImagingEx Vivo Slice Culture ProtocolWide-field Fluorescence MicroscopyTime-lapse MonitoringSpatial ResolutionTemporal ResolutionCellular BehaviorsSub-cellular BehaviorsGene Activity ReportersNotch Signaling

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Cite this Article

Das, R. M., Wilcock, A. C., Swedlow, J. R., Storey, K. G. High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium. J. Vis. Exp. (62), e3920, doi:10.3791/3920 (2012).

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