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High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium
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High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium
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10:59 min
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April 12, 2012
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Imaging embryonic tissue in real-time is challenging over long periods of time. Here we present an assay for monitoring cellular and sub-cellular changes in chick spinal cord for long periods with high spatial and temporal resolution. This technique can be adapted for other regions of the nervous system and developing embryo.
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High-resolutionLive ImagingCell BehaviorDeveloping NeuroepitheliumEmbryonic Spinal CordCycling Neural Progenitor CellsNeuronal DifferentiationMolecular MechanismsNeurogenesisReal-time ImagingCell ViabilityCulturePhototoxic EffectsFluorescent ImagingEx Vivo Slice Culture ProtocolWide-field Fluorescence MicroscopyTime-lapse MonitoringSpatial ResolutionTemporal ResolutionCellular BehaviorsSub-cellular BehaviorsGene Activity ReportersNotch Signaling
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Das, R. M., Wilcock, A. C., Swedlow, J. R., Storey, K. G. High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium. J. Vis. Exp. (62), e3920, doi:10.3791/3920 (2012).
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