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热酚水提取纯化及革兰氏阴性菌脂多糖的可视化

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

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热酚水提取纯化及革兰氏阴性菌脂多糖的可视化

DOI:

•

05:31 min

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May 28, 2012

•

Michael R. Davis, Jr., Joanna B. Goldberg

¹Department of Microbiology, Immunology, & Cancer Biology,University of Virginia Health System

Capítulos

00:05Título
01:38Preparation of Bacteria and LPS Extraction
04:21LPS Purification Results
04:48Conclusion

Summary

Tadução automática

我们描述一个净化革兰氏阴性菌脂多糖（LPS）的修改热水溶液中苯酚的提取方法。一旦提取，内毒素可随后通过SDS-PAGE分析，并通过可视化的直接染色或西方免疫。

Tags

Purification Visualization Lipopolysaccharide Gram-negative Bacteria Hot Aqueous-phenol Extraction Lipid A Core Oligosaccharide O-antigen Units Virulence Pathogenesis Serotyping Clinical Isolates Antibodies Mutants Extraction Method Nucleic Acids Proteins Sodium Dodecy

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