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Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

33,909 Views

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05:31 min

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May 28, 2012

DOI:

10.3791/3916-v

DOI:
10.3791/3916-v

•

05:31 min
May 28, 2012

•

15 Views
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1Department of Microbiology, Immunology, & Cancer Biology,University of Virginia Health System

Tags

PurificationVisualizationLipopolysaccharideGram-negative BacteriaHot Aqueous-phenol ExtractionLipid ACore OligosaccharideO-antigen UnitsVirulencePathogenesisSerotypingClinical IsolatesAntibodiesMutantsExtraction MethodNucleic AcidsProteinsSodium Dodecy

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Davis, Jr., M. R., Goldberg, J. B. Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction. J. Vis. Exp. (63), e3916, doi:10.3791/3916 (2012).

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