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Zuivering en Visualisatie van Lipopolysaccharide van Gram-negatieve bacteriën door Hot Waterige-fenolextractie
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
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Zuivering en Visualisatie van Lipopolysaccharide van Gram-negatieve bacteriën door Hot Waterige-fenolextractie

34,039 Views

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05:31 min

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May 28, 2012

DOI:

10.3791/3916-v

DOI:
10.3791/3916-v

•

05:31 min
May 28, 2012

•

25 Views
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1Department of Microbiology, Immunology, & Cancer Biology,University of Virginia Health System

Summary

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Wij beschrijven een gemodificeerd hot waterige fenol extractie werkwijze voor het zuiveren lipopolysaccharide (LPS) van Gram-negatieve bacteriën. Afgetapt kan de LPS vervolgens geanalyseerd door SDS-PAGE en gevisualiseerd door directe kleuring of Western immunoblot.

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Davis, Jr., M. R., Goldberg, J. B. Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction. J. Vis. Exp. (63), e3916, doi:10.3791/3916 (2012).

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