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从使用鸡蛋<em>血吸虫</em>作为<em>在体内</em>蠕虫引起的肺部炎症模型
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
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从使用鸡蛋<em>血吸虫</em>作为<em>在体内</em>蠕虫引起的肺部炎症模型

DOI:
10.3791/3905-v

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09:58 min

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June 05, 2012

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, , ,
1Institute of Immunology, Department of Microbiology, Perelman School of Medicine,University of Pennsylvania , 2Pathobiology, School of Veterinary Medicine,University of Pennsylvania

Capítulos

  • 00:05Título
  • 01:25Purifying Schistosoma mansoni Eggs
  • 04:00Intraperitoneal Sensitization with S. mansoni Eggs: Day 0
  • 04:34Intravenous Challenge with S. mansoni Eggs: Day 14
  • 05:06Experimental Harvest: Day 22
  • 07:06Preparation of Recovered Tissues
  • 07:53Results: Inflammatory Response to S. mansoni Infection
  • 09:31Conclusion

Summary

Tadução automática

Tadução automática

血吸虫卵是强有力的刺激T辅助2型（Th2细胞）的免疫反应，寄生虫感染的特点，哮喘和过敏性炎症。该协议利用S。血吸虫卵注射产生的CD4 Th2细胞因子诱导的炎症反应在肺，肺肉芽肿形成的特点，各地的鸡蛋，嗜酸性粒细胞和巨噬细胞替代激活。

Tags

Schistosoma MansoniHelminth-induced Lung InflammationParasite EggsImmune ResponseTh2 ResponseIL-4IL-5IL-13Alternative Activation Of MacrophagesEosinophilsPulmonary GranulomasAirway InflammationIntraperitoneal SensitizationIntravenous ChallengeLung ParenchymaLymphocytesCD4 T Cell ActivationIsolating Parasite EggsBroncho-alveolar Lavage (BAL)Analysis

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