/
/
Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
13,658 Views
•
09:58 min
•
June 05, 2012
DOI:
Summary
Automatically generated
Schistosoma mansoni eggs are potent stimulators of the T helper type 2 (Th2) immune response, characteristic of parasite infection, asthma and allergic inflammation. This protocol utilizes S. mansoni egg injection to generate a CD4 Th2 cytokine-induced inflammatory response in the lung, characterized by lung granuloma formation around the egg, eosinophilia and macrophage alternative activation.
Explore More Videos
Schistosoma MansoniHelminth-induced Lung InflammationParasite EggsImmune ResponseTh2 ResponseIL-4IL-5IL-13Alternative Activation Of MacrophagesEosinophilsPulmonary GranulomasAirway InflammationIntraperitoneal SensitizationIntravenous ChallengeLung ParenchymaLymphocytesCD4 T Cell ActivationIsolating Parasite EggsBroncho-alveolar Lavage (BAL)Analysis
Read Article
Cite this Article
Joyce, K. L., Morgan, W., Greenberg, R., Nair, M. G. Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation. J. Vis. Exp. (64), e3905, doi:10.3791/3905 (2012).
Copy