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Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
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June 05, 2012
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Schistosoma mansoni eggs are potent stimulators of the T helper type 2 (Th2) immune response, characteristic of parasite infection, asthma and allergic inflammation. This protocol utilizes S. mansoni egg injection to generate a CD4 Th2 cytokine-induced inflammatory response in the lung, characterized by lung granuloma formation around the egg, eosinophilia and macrophage alternative activation.
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Schistosoma MansoniHelminth-induced Lung InflammationParasite EggsImmune ResponseTh2 ResponseIL-4IL-5IL-13Alternative Activation Of MacrophagesEosinophilsPulmonary GranulomasAirway InflammationIntraperitoneal SensitizationIntravenous ChallengeLung ParenchymaLymphocytesCD4 T Cell ActivationIsolating Parasite EggsBroncho-alveolar Lavage (BAL)Analysis
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Joyce, K. L., Morgan, W., Greenberg, R., Nair, M. G. Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation. J. Vis. Exp. (64), e3905, doi:10.3791/3905 (2012).
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