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Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

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14:23 min

April 16, 2012

DOI:

14:23 min
April 16, 2012

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Summary

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We describe a method for generating regulatory, memory and naïve T cells from a single human blood donor. Polarized Tregs can be then compared to other subsets in a variety of genetic and functional applications with genetic homogeneity, including a suppression assay also detailed here.

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