需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
23,792 Views
•
14:23 min
•
April 16, 2012
DOI:
Summary
Automatically generated
We describe a method for generating regulatory, memory and naïve T cells from a single human blood donor. Polarized Tregs can be then compared to other subsets in a variety of genetic and functional applications with genetic homogeneity, including a suppression assay also detailed here.
Explore More Videos
Induced Regulatory T CellsPrimary Human Naive T CellsMemory T CellsImmunosuppressive CD4+ Regulatory T CellsPeripheral ToleranceImmunologic HomeostasisSelf AntigensCommensal AntigensTreg BiologyTherapeutic Injection Of TregsMurine ModelsColitisType I DiabetesRheumatoid ArthritisGraft Versus Host DiseaseEx Vivo ExpansionPeripheral Blood Mononuclear CellsCD4+ T CellsIn Vitro Cell CultureFluorescence Activated Cell Sorting (FACS)Forkhead Box P3 (FoxP3)Activation-induced Transcription Factor
Read Article
Cite this Article
Ellis, G. I., Reneer, M. C., Vélez-Ortega, A. C., McCool, A., Martí, F. Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells. J. Vis. Exp. (62), e3738, doi:10.3791/3738 (2012).
Copy