ジャーナル
/
免疫学と感染
/
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

DOI:
10.3791/3738-v

•

14:23 min

•

April 16, 2012

•

, , , ,
1Department of Microbiology, Immunology and Molecular Genetics,University of Kentucky

章

  • 00:05標題
  • 02:22Isolation of Human Peripheral Blood Mononuclear Cells (PBMCs) from Buffy Coat
  • 03:58Magnetic Negative Selection of T Cells from PBMCs using EasySep Enrichment Kit
  • 06:16Cell Culture Conditions to Induce Regulatory T Cells
  • 07:11Fluorescence-activated Cell Sorting of Three Populations of T Cells
  • 08:28Suppression Assay
  • 10:32Representative Sorting and Suppression Assay Results
  • 13:14Conclusion

概要

自動翻訳

自動翻訳

We describe a method for generating regulatory, memory and naïve T cells from a single human blood donor. Polarized Tregs can be then compared to other subsets in a variety of genetic and functional applications with genetic homogeneity, including a suppression assay also detailed here.

タグ

Induced Regulatory T CellsPrimary Human Naive T CellsMemory T CellsImmunosuppressive CD4+ Regulatory T CellsPeripheral ToleranceImmunologic HomeostasisSelf AntigensCommensal AntigensTreg BiologyTherapeutic Injection Of TregsMurine ModelsColitisType I DiabetesRheumatoid ArthritisGraft Versus Host DiseaseEx Vivo ExpansionPeripheral Blood Mononuclear CellsCD4+ T CellsIn Vitro Cell CultureFluorescence Activated Cell Sorting (FACS)Forkhead Box P3 (FoxP3)Activation-induced Transcription Factor

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes

piggyBac Transposon System Modification of Primary Human T Cells

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

T Cells Capture Bacteria by Transinfection from Dendritic Cells

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Read Article