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杂交<em>原位</em>和蚊虫唾液腺，卵巢，胚胎
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
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杂交<em>原位</em>和蚊虫唾液腺，卵巢，胚胎

DOI:
10.3791/3709-v

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10:58 min

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June 28, 2012

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,
1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Department of Microbiology and Molecular Genetics,University of California, Irvine

Capítulos

  • 00:05Título
  • 01:25In situ Hybridization
  • 03:27Glycerol Mounting
  • 05:30Dechorionation, Fixation, and Endochorion Disruption
  • 08:04Peeling
  • 09:44Representative In situ Resultados
  • 10:36Conclusion

Summary

Tadução automática

Tadução automática

时间和空间上的基因表达分析在功能基因组学的重要作用。全挂载杂交<em>原位</em>是用于确定本地化组织和亚细胞车厢内的成绩单。在这里，我们勾勒1杂交<em>原位</em>蚊子的具体目标组织的修改协议。

Tags

Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization

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