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Imunologia e Infecção
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Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

DOI:
10.3791/3709-v

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10:58 min

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June 28, 2012

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,
1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Department of Microbiology and Molecular Genetics,University of California, Irvine

Capítulos

  • 00:05Título
  • 01:25In situ Hybridization
  • 03:27Glycerol Mounting
  • 05:30Dechorionation, Fixation, and Endochorion Disruption
  • 08:04Peeling
  • 09:44Representative In situ Resultados
  • 10:36Conclusion

Summary

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Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.

Tags

Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization

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