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检测内源NADH的荧光双光子成像的小鼠大脑皮层Microregional缺氧

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

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检测内源NADH的荧光双光子成像的小鼠大脑皮层Microregional缺氧

DOI:

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12:03 min

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February 21, 2012

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Oksana Polesskaya, Anita Sun, Gheorghe Salahura, Jharon N. Silva, Stephen Dewhurst, Karl Kasischke

¹Department of Microbiology and Immunology,University of Rochester Medical Center, ²Center for Neural Development and Disease,University of Rochester Medical Center, ³Deptartment of Neurology, Center for Neural Development and Disease,University of Rochester Medical Center

Capítulos

00:05Título
01:42Preparing the Animal for IMaging
02:56Preparing the Open Skull Cranial Window
06:40Intravenous Injection and Blood Oxygenation Monitoring
07:58Two-photon Imaging
09:30Representative Hypoxia Measurement Results
11:14Conclusion

Summary

Tadução automática

在这里，我们描述了一个方法，直接可视化在小鼠皮层microregional组织缺氧<em>在体内</em>。它是基于并发双光子成像烟酰胺腺嘌呤二核苷酸（NADH）和皮质微循环。这种方法是有用的组织供氧的高分辨率分析。

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