Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

Oksana Polesskaya; Anita Sun; Gheorghe Salahura; Jharon N. Silva; Stephen Dewhurst; Karl Kasischke

doi:10.3791/3466

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Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

15,849 Views

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12:03 min

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February 21, 2012

DOI:

10.3791/3466-v

DOI:

10.3791/3466-v

•

12:03 min

February 21, 2012

•

6 Views

Oksana Polesskaya¹, Anita Sun², Gheorghe Salahura², Jharon N. Silva¹, Stephen Dewhurst¹, Karl Kasischke³

¹Department of Microbiology and Immunology,University of Rochester Medical Center, ²Center for Neural Development and Disease,University of Rochester Medical Center, ³Deptartment of Neurology, Center for Neural Development and Disease,University of Rochester Medical Center

Summary

Here we describe a method to directly visualize microregional tissue hypoxia in the mouse cortex in vivo. It is based on concurrent two-photon imaging of nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation. This method is useful for high resolution analysis of tissue oxygen supply.

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Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

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