Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

Oksana Polesskaya; Anita Sun; Gheorghe Salahura; Jharon N. Silva; Stephen Dewhurst; Karl Kasischke

doi:10.3791/3466

Journal

/

Neurosciences

/

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

Journal JoVE
Neurosciences

This content is Free Access.

Journal JoVE Neurosciences

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

15,849 Views

•

12:03 min

•

February 21, 2012

DOI:

10.3791/3466-v

DOI:

10.3791/3466-v

•

12:03 min

February 21, 2012

•

6 Views

Oksana Polesskaya¹, Anita Sun², Gheorghe Salahura², Jharon N. Silva¹, Stephen Dewhurst¹, Karl Kasischke³

¹Department of Microbiology and Immunology,University of Rochester Medical Center, ²Center for Neural Development and Disease,University of Rochester Medical Center, ³Deptartment of Neurology, Center for Neural Development and Disease,University of Rochester Medical Center

Summary

Here we describe a method to directly visualize microregional tissue hypoxia in the mouse cortex in vivo. It is based on concurrent two-photon imaging of nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation. This method is useful for high resolution analysis of tissue oxygen supply.

Read Article

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

Summary

Automatically generated

Explore More Videos