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Imunologia e Infecção
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Murine Superficial Lymph Node Surgery
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Murine Superficial Lymph Node Surgery

Murine Superficial Lymph Node Surgery

DOI:
10.3791/3444-v

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04:36 min

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May 21, 2012

•

,
1Maisonneuve-Rosemont Hospital Research Center, 2Department of Microbiology and Immunology,University of Montreal, 3Department of Medicine,University of Montreal

Capítulos

  • 00:05Título
  • 00:53Murine Superficial Lymph Node Surgery
  • 03:53Results: LN Surgery is a Viable Method for Harvesting Lymphocytes
  • 04:19Conclusion

Summary

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To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.

Tags

MurineSuperficial Lymph Node SurgeryImmunologyImmune ResponseVaccineInfectionTumorLymphocyte HomeostasisTime Course ExperimentsExperimental VariabilityBlood WithdrawalCirculating LymphocytesLymph NodesSurgeryImmune Cell ExpansionPhenotypic CharacterizationFunctional AssaysBrachial Lymph NodesInguinal Lymph NodesSmall Incision In The SkinAnesthetized MouseQuick RecoveryMinimal Pain

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