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Murine Superficial Lymph Node Surgery
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04:36 min
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May 21, 2012
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Summary
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To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.
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MurineSuperficial Lymph Node SurgeryImmunologyImmune ResponseVaccineInfectionTumorLymphocyte HomeostasisTime Course ExperimentsExperimental VariabilityBlood WithdrawalCirculating LymphocytesLymph NodesSurgeryImmune Cell ExpansionPhenotypic CharacterizationFunctional AssaysBrachial Lymph NodesInguinal Lymph NodesSmall Incision In The SkinAnesthetized MouseQuick RecoveryMinimal Pain
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Cite this Article
Mathieu, M., Labrecque, N. Murine Superficial Lymph Node Surgery. J. Vis. Exp. (63), e3444, doi:10.3791/3444 (2012).
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