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Murine Superficial Lymph Node Surgery
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JoVE Journal 免疫学と感染
Murine Superficial Lymph Node Surgery

Murine Superficial Lymph Node Surgery

DOI:
10.3791/3444-v

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04:36 min

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May 21, 2012

•

,
1Maisonneuve-Rosemont Hospital Research Center, 2Department of Microbiology and Immunology,University of Montreal, 3医学部,University of Montreal

章

  • 00:05標題
  • 00:53Murine Superficial Lymph Node Surgery
  • 03:53Results: LN Surgery is a Viable Method for Harvesting Lymphocytes
  • 04:19Conclusion

概要

自動翻訳

自動翻訳

To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.

タグ

MurineSuperficial Lymph Node Surgery免疫学Immune ResponseVaccineInfectionTumorLymphocyte HomeostasisTime Course ExperimentsExperimental VariabilityBlood WithdrawalCirculating LymphocytesLymph NodesSurgeryImmune Cell ExpansionPhenotypic CharacterizationFunctional AssaysBrachial Lymph NodesInguinal Lymph NodesSmall Incision In The SkinAnesthetized MouseQuick RecoveryMinimal Pain

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