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重组腺相关病毒载体的生产和滴定
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Production and Titering of Recombinant Adeno-associated Viral Vectors
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重组腺相关病毒载体的生产和滴定

60,739 Views

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08:35 min

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November 27, 2011

DOI:

10.3791/3348-v

DOI:
10.3791/3348-v

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08:35 min
November 27, 2011

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1School of Medical Sciences, College of Life Sciences and Medicine,University of Aberdeen, 2Translational Neuroscience Facility and Department of Physiology,School of Medical Sciences, University of New South Wales, 3Department of Biochemistry and Molecular Biophysics,Columbia University

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Recombinant Adeno-associated Viral VectorsAAVIn Vivo StudiesHuman Clinical TrialsTransduction ProfileImmune ResponseTransgene ExpressionGene DeliveryRAAVsSafety ProfileChimeric RAAVsAAV1AAV2Affinity ChromatographyInfectious PropertiesHeparin Column PurificationViral Titer

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McClure, C., Cole, K. L. H., Wulff, P., Klugmann, M., Murray, A. J. Production and Titering of Recombinant Adeno-associated Viral Vectors. J. Vis. Exp. (57), e3348, doi:10.3791/3348 (2011).

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