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发展的细胞类型特异性的抗艾滋病毒gp120基因的siRNA传递适配
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
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发展的细胞类型特异性的抗艾滋病毒gp120基因的siRNA传递适配

DOI:
10.3791/2954-v

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13:47 min

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June 23, 2011

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, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Shared Resource-DNA/RNA Peptide,Beckman Research Institute of City of Hope

Capítulos

  • 00:05Título
  • 01:20Preparation of RNA Library
  • 03:00In vitro Generation of Aptamers by Selex
  • 06:47Monitoring SELEX Progress
  • 09:51Isolation and Characterization of New RNA Aptamers Against HIV-1BaL gp120
  • 13:27Conclusion

Summary

Tadução automática

Tadução automática

从RNA库是孤立的几个2' -氟RNA对HIV - 1BA - L的gp120的nanomole亲和力的适配<em>在体外</em> SELEX程序。创建一种新的双抑菌功能抗- gp120的适配子的siRNA的嵌合体，显示了相当的全身抗艾滋病毒治疗的承诺。

Tags

Cell-type SpecificAnti-HIVGp120 AptamersSiRNA DeliveryHIV InfectionAntiretroviral AgentsSmall Interfering (si)RNAsTherapeuticsRNAi-basedHIV/AIDSRNA TranscriptsEfficient DeliveryTargeted Intracellular SiRNA Delivery ApproachHIV-1 Gp120 ProteinGlycoprotein EnvelopeViral EntryCD4 CellsAnti-viral StrategyDrug Discovery2′-F Modified Anti-HIV Gp120 RNA AptamersNitrocellulose Filter SELEX Method

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