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Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
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June 23, 2011
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Several 2’-Fluoro RNA aptamers against HIV-1Ba-L gp120 with nanomole affinity are isolated from a RNA library by in vitro SELEX procedure. A new dual inhibitory function anti-gp120 aptamer-siRNA chimera is created and shows considerable promise for systemic anti-HIV therapy.
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Cell-type SpecificAnti-HIVGp120 AptamersSiRNA DeliveryHIV InfectionAntiretroviral AgentsSmall Interfering (si)RNAsTherapeuticsRNAi-basedHIV/AIDSRNA TranscriptsEfficient DeliveryTargeted Intracellular SiRNA Delivery ApproachHIV-1 Gp120 ProteinGlycoprotein EnvelopeViral EntryCD4 CellsAnti-viral StrategyDrug Discovery2′-F Modified Anti-HIV Gp120 RNA AptamersNitrocellulose Filter SELEX Method
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Zhou, J., Li, H., Zhang, J., Piotr, S., Rossi, J. Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery. J. Vis. Exp. (52), e2954, doi:10.3791/2954 (2011).
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