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En snabb metod för högupplöst fluorescens avbildning i semi-Tjock hjärnan skivor
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Neurociência
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JoVE Journal Neurociência
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
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En snabb metod för högupplöst fluorescens avbildning i semi-Tjock hjärnan skivor

16,093 Views

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04:35 min

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July 26, 2011

DOI:

10.3791/2807-v

DOI:
10.3791/2807-v

•

04:35 min
July 26, 2011

•

5 Views
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1Department of Molecular & Human Genetics,Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience,Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute,Texas Children's Hospital

Summary

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Här beskriver vi en snabb och enkel metod för att bilden fluorescerande celler i semi-tjocka hjärnan skivor. Genom att fastställa, skivning, och optiskt clearing hjärnvävnad beskriver vi hur standarden epifluorescent eller konfokal avbildning kan användas för att visualisera enskilda celler och neuronala nätverk inom intakt nervvävnad.

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Cite this Article

Selever, J., Kong, J., Arenkiel, B. R. A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices. J. Vis. Exp. (53), e2807, doi:10.3791/2807 (2011).

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