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In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
Capítulos
- 00:05Título
- 02:01Spinal Cord Surgery
- 04:36Stabilization of the Spinal Column and Preparation for in vivo Imaging
- 06:10In vivo Imaging of the Mouse Spinal Cord with Two-photon Microscopy
- 07:16Representative Results for in vivo Imaging of the Mouse Spinal Cord
- 09:54Conclusion
A minimally invasive protocol to stabilize the mouse spinal column and perform repetitive in vivo spinal cord imaging using two-photon microscopy is described. This method combines a spinal stabilization device and an anesthetic regimen to minimize respiratory-induced movements and produce raw imaging data that require no alignment or other post-processing.
Tags
In Vivo ImagingTwo-photon MicroscopyMouse Spinal CordFluorescent ProteinsSpecific Cell TypesPhysiological ProcessesPathological ProcessesCentral Nervous SystemBrain ImagingNeuronsAstrocytesMicrogliaTechnical ComplicationsSpinal Cord ImagingMovement ArtifactSpinal Stabilization DeviceDeep Anesthesia Protocol
