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In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
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JoVE Journal Neuroscienze
In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

23,480 Views

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10:24 min

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January 05, 2012

DOI:

10.3791/2760-v

DOI:
10.3791/2760-v

•

10:24 min
January 05, 2012

•

18 Views
,
1Gladstone Institute of Neurological Disease,University of California, San Francisco , 2Department of Neurology,University of California, San Francisco

Summary

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A minimally invasive protocol to stabilize the mouse spinal column and perform repetitive in vivo spinal cord imaging using two-photon microscopy is described. This method combines a spinal stabilization device and an anesthetic regimen to minimize respiratory-induced movements and produce raw imaging data that require no alignment or other post-processing.

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Cite this Article

Davalos, D., Akassoglou, K. In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy. J. Vis. Exp. (59), e2760, doi:10.3791/2760 (2012).

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