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JoVE Journal Neuroscience
In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
 

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

Article DOI: 10.3791/2760-v • 10:24 min • January 5th, 2012
January 5th, 2012 •
1, 1,2
1Gladstone Institute of Neurological Disease, University of California, San Francisco, 2Department of Neurology, University of California, San Francisco

Kapitel

Summary
Automatic Translation

A minimally invasive protocol to stabilize the mouse spinal column and perform repetitive in vivo spinal cord imaging using two-photon microscopy is described. This method combines a spinal stabilization device and an anesthetic regimen to minimize respiratory-induced movements and produce raw imaging data that require no alignment or other post-processing.

Tags

In Vivo Imaging Two-photon Microscopy Mouse Spinal Cord Fluorescent Proteins Specific Cell Types Physiological Processes Pathological Processes Central Nervous System Brain Imaging Neurons Astrocytes Microglia Technical Complications Spinal Cord Imaging Movement Artifact Spinal Stabilization Device Deep Anesthesia Protocol
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