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In kaart brengen en de toepassing van Enhancer-trap Flippase Expression in larvale en volwassen Drosophila CNS
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
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In kaart brengen en de toepassing van Enhancer-trap Flippase Expression in larvale en volwassen Drosophila CNS

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09:45 min

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June 03, 2011

DOI:

10.3791/2649-v

DOI:
10.3791/2649-v

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09:45 min
June 03, 2011

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3 Views
, , , , , , , ,
1Department of Zoology,University of Oklahoma – Norman, 2Department of Biology,Brandeis University

Summary

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Beschrijven we een Flippase-geïnduceerde intersectionele Gal80/Gal4 repressie (FINGR)-methode, waardoor weefsel-specifieke Forever om Gal80 expressiepatronen te bepalen. Waar Gal4 en FLP elkaar overlappen, is Gal4 uitdrukking ingeschakeld (Gal80 omgedraaid out) of uit (Gal80 gespiegeld in). De FINGR methode is veelzijdig voor klonale analyse en neurale circuit in kaart brengen.

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Fore, T. R., Ojwang, A. A., Warner, M. L., Peng, X., Bohm, R. A., Welch, W. P., Goodnight, L. K., Bao, H., Zhang, B. Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS. J. Vis. Exp. (52), e2649, doi:10.3791/2649 (2011).

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