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斑马鱼化工屏幕自动成像和分析的发展。

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Development of automated imaging and analysis for zebrafish chemical screens.

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斑马鱼化工屏幕自动成像和分析的发展。

11,146 Views

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10:49 min

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June 24, 2010

DOI:

DOI:

•

10:49 min

June 24, 2010

•

1 Views

Andreas Vogt¹, Hiba Codore², Billy W. Day^3,4, Neil A. Hukriede², Michael Tsang²

¹Pharmacology and Chemical Biology,University of Pittsburgh Drug Discovery Institute, ²Department of Microbiology and Molecular Genetics,University of Pittsburgh, ³Department of Pharmaceutical Sciences,University of Pittsburgh, ⁴Department of Chemistry,University of Pittsburgh

Summary

我们报告的自动成像系统和分析在多孔板的转基因斑马鱼的胚胎的发展。这表明成纤维细胞生长因子记者的基因表达的能力来衡量的一个小分子，BCI，剂量依赖效应，并提供技术建立高通量的斑马鱼化工屏幕。

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