Journal
/
Biologia
/
Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
JoVE Journal
Biologia
Author Produced
This content is Free Access.
JoVE Journal Biologia
Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

DOI:
10.3791/1690-v

•

08:19 min

•

February 16, 2010

•

, , , , , ,
1Department of Environment and Agro-Biotechnologies (EVA), Nutrition and Toxicology Unit (NuTox),Centre de Recherche Public-Gabriel Lippmann

Capítulos

  • 00:05Título
  • 08:15Credits

Summary

Tadução automática

Tadução automática

Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.

Tags

Toxin InductionProtein ExtractionFusarium Spp.Proteomic StudiesMycotoxinsPathogenicity FactorsProtein ExpressionStandardizationBiological ReplicatesRobustness Of DataExtraction ProcedureCulture GrowthSolid Media

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

Electrophoretic Separation of Proteins

Interview: Protein Folding and Studies of Neurodegenerative Diseases

Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies

Digital Microfluidics for Automated Proteomic Processing

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Sexual Development and Ascospore Discharge in Fusarium graminearum

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Read Article