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生物学
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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
JoVE 杂志
生物学
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JoVE 杂志 生物学
Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

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08:19 min

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February 16, 2010

DOI:

10.3791/1690-v

DOI:
10.3791/1690-v

•

08:19 min
February 16, 2010

•

2 Views
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1Department of Environment and Agro-Biotechnologies (EVA), Nutrition and Toxicology Unit (NuTox),Centre de Recherche Public-Gabriel Lippmann

Summary

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Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.

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Cite this Article

Pasquali, M., Giraud, F., Lasserre, J. P., Planchon, S., Hoffmann, L., Bohn, T., Renaut, J. Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies. J. Vis. Exp. (36), e1690, doi:10.3791/1690 (2010).

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