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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
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生物学
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JoVE Journal 生物学
Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

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08:19 min

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February 16, 2010

DOI:

10.3791/1690-v

DOI:
10.3791/1690-v

•

08:19 min
February 16, 2010

•

2 Views
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1Department of Environment and Agro-Biotechnologies (EVA), Nutrition and Toxicology Unit (NuTox),Centre de Recherche Public-Gabriel Lippmann

概要

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Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.

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Cite this Article

Pasquali, M., Giraud, F., Lasserre, J. P., Planchon, S., Hoffmann, L., Bohn, T., Renaut, J. Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies. J. Vis. Exp. (36), e1690, doi:10.3791/1690 (2010).

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