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Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
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Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

DOI:

06:39 min

June 30, 2023

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Chapters

  • 00:00Introduction
  • 00:44Flow Cytometry Setting and Preparation of Autoclaved Spores for Flow Cytometry
  • 01:47Analysis Using Flow Cytometry
  • 03:27Estimation of the Protein Coupling Index on a Spore Surface Using Flow Cytometry
  • 04:52Results: Fluorescence Coupling Percentage Analysis of Bacillus subtilis Spores
  • 06:08Conclusion

Summary

자동 번역

This protocol focuses on the use of flow cytometry and counting beads to quantify bacterial spores labeled with ethidium bromide. The method is also efficient for analyzing the covalent coupling of proteins on the surface of intact spores.

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