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El colgajo axial superficial de la arteria epigástrica inferior para estudiar los efectos del preacondicionamiento isquémico en un modelo de rata
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Vascularized Composite Allotransplantation Laboratory,Massachusetts General Hospital, 2Harvard Medical School, 3Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University Hospital Center (CHU de Rennes),University of Rennes 1, 4Plastic Surgery Research Laboratory,Massachusetts General Hospital, 5Shriners Children’s Boston, 6Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, 7Department of Plastic Surgery and Hand Surgery,University Medical Center Utrecht, 8Department of Plastic, Reconstructive, and Aesthetic Surgery, Groupe Almaviva Santé,Clinique de l’Alma
Chapters
- 00:05Introduction
- 00:56Harvesting the Epigastric Flap
- 03:43Vessel Preparation and Ischemia Induction
- 06:42Results: Analyzing Post-Operative Flap Viability
- 08:19Conclusion
Este protocolo describe la recolección, sutura y monitoreo de colgajos fasciocutáneos en ratas que permiten una buena visualización y manipulación del flujo sanguíneo a través de los vasos epigástricos inferiores superficiales mediante el pinzamiento y la ligadura de los vasos femorales. Esto es crítico para los estudios que involucran preacondicionamiento isquémico.
