The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model

Yanis Berkane; Austin Alana Shamlou; Jose Reyes; Hyshem H. Lancia; Irina Filz von Reiterdank; Nicolas Bertheuil; Basak E. Uygun; Korkut Uygun; William G. Austen Jr.; Curtis L. Cetrulo Jr.; Mark A. Randolph; Alexandre  G. Lellouch

doi:10.3791/64980

Journal

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Imunologia e Infecção

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The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model

1,315 Views

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09:07 min

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January 27, 2023

DOI:

10.3791/64980-v

DOI:

10.3791/64980-v

•

09:07 min

January 27, 2023

•

5 Views

Yanis Berkane*^1,2,3,5, Austin Alana Shamlou*^1,2,4,5, Jose Reyes^1,2, Hyshem H. Lancia^1,2,5, Irina Filz von Reiterdank^1,2,5,6,7, Nicolas Bertheuil³, Basak E. Uygun^2,5,6, Korkut Uygun^1,2,5,6, William G. Austen Jr.^2,4, Curtis L. Cetrulo Jr.^1,2,4,5, Mark A. Randolph^1,2,4,5, Alexandre G. Lellouch^1,2,5,6,8

¹Vascularized Composite Allotransplantation Laboratory,Massachusetts General Hospital, ²Harvard Medical School, ³Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University Hospital Center (CHU de Rennes),University of Rennes 1, ⁴Plastic Surgery Research Laboratory,Massachusetts General Hospital, ⁵Shriners Children’s Boston, ⁶Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, ⁷Department of Plastic Surgery and Hand Surgery,University Medical Center Utrecht, ⁸Department of Plastic, Reconstructive, and Aesthetic Surgery, Groupe Almaviva Santé,Clinique de l’Alma

Summary

This protocol describes harvesting, suturing, and monitoring fasciocutaneous flaps in rats that allow for good visualization and manipulation of blood flow through the superficial inferior epigastric vessels by means of clamping and ligating the femoral vessels. This is critical for studies involving ischemic preconditioning.

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The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model

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