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Lentiviral Vector Preparation for In Vivo Gene Modulation
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Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation
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Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in Mice

Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation

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00:55 min

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February 16, 2024

DOI:

10.3791/64926-v

DOI:
10.3791/64926-v

•

00:55 min
February 16, 2024

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29 Views
, , , , , , ,
1Systems Immunity Research Institute, Division of Infection and Immunity,Cardiff University, 2Biomedical Sciences Unit, Faculty of Medicine, Health and Life Science,Swansea University, 3UK Dementia Research Institute

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Lentiviral VectorGene ModulationMicroRNA ModulationPeritoneal MacrophagesIn VivoHomeostasisPeritonitisHEK293T CellsSucrose PurificationIntraperitoneal InjectionGene Function

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Gurney, M., Davies, L. C., Jones, R. E., Bart, V. M., Jenkins, R. H., Brennan, P., Taylor, P. R., Czubala, M. A. Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in Mice. J. Vis. Exp. (204), e64926, doi:10.3791/64926 (2024).

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