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使用胚胎海马神经元冷冻原液进行简单且可重复的低密度原代培养
JoVE 신문
신경과학
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JoVE 신문 신경과학
Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons
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使用胚胎海马神经元冷冻原液进行简单且可重复的低密度原代培养

DOI:
10.3791/64872-v

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04:26 min

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January 27, 2023

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, , , , ,
1Department of Pharmacology, Graduate School of Medicine,Gunma University, 2Gunma University Initiative for Advanced Research,Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences,The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc.

Chapters

  • 00:05Introduction
  • 00:36Plate Coating and Cell Seeding
  • 02:39Neuronal Treatments and Fixation
  • 03:33Results: Neuronal Low-Density Primary Culture Assessment
  • 03:58Conclusion

Summary

자동 번역

자동 번역

即用型冷冻神经元库存是评估突触功能的有力工具。在这里，我们使用96孔板从冷冻原液中引入一种简单的低密度原代培养物。

Tags

Low-density Primary CultureFrozen StockEmbryonic Hippocampal NeuronsCell CulturePoly-L-lysineMicroelectrode ArrayCell SeedingCell SuspensionCytosine Beta-D-arabinofuranosideGlial Cell Growth

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