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Immunology and Infection

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JoVE Journal Immunology and Infection
Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo
 

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Article DOI: 10.3791/64742-v • 06:29 min • May 5th, 2023
May 5th, 2023 •
*1, *1, 1
1Department of Basic Medicine, Wuxi School of Medicine, Jiangnan University
* These authors contributed equally

챕터

요약
Automatic Translation

This protocol describes the isolation of pulmonary interstitial macrophages (IMs) and their adoptive transfer after IL-33 stimulation of the lung alveoli in a mouse model, which can facilitate the in vivo study of idiopathic pulmonary fibrosis (IPF).

Tags

Adoptive Transfer IL-33-stimulated Macrophages Bleomycin-induced Mouse Models Idiopathic Pulmonary Fibrosis In Vivo Study Isolation Of Pulmonary IMS Traditional Cytokines Clodronate Liposomes Nasal Cavity Administration Depletion Of Alveolar Macrophages Disinfection Of Skin Cardiopulmonary Tissue Exposure PBS Perfusion
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