Journal
/
면역학 및 감염병학
/
A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis
Please note that all translations are automatically generated. Click here for the English version.

A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

DOI:
10.3791/64634-v

•

05:44 min

•

December 09, 2022

•

1The Rausing Laboratory, Autoimmunity Section, Division of Neurosurgery, Department of Clinical Sciences,Lund University, 2Department of Autoimmunity,BTB Emulsions

Chapters

  • 00:04Introduction
  • 00:59Material Preparation
  • 01:38Preparation of CFA/Peptide Emulsions
  • 03:31Quality Control of Emulsions
  • 04:01Results: Characterization of Emulsions Produced and EAE Induction in Immunized Mice
  • 05:02Conclusion

Summary

자동 번역

자동 번역

To induce experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis, mice are immunized with a water-in-oil emulsion containing an autoantigen and complete Freund's adjuvant. While several protocols exist for the preparation of these emulsions, a rapid, simple, and standardized homogenization protocol for emulsion preparation is presented here.

Tags

Homogenization MethodAntigen/adjuvant EmulsionExperimental Autoimmune EncephalomyelitisEAECFA SlurryIFA AdjuvantEmulsion PreparationAutoimmune Disease ModelCancer Vaccines

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/64634?article_type=v

Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis
check_url/kr/64634?article_type=v

Induction of Experimental Autoimmune Hypophysitis in SJL Mice
check_url/kr/64634?article_type=v

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
check_url/kr/64634?article_type=v

Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice
check_url/kr/64634?article_type=v

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
check_url/kr/64634?article_type=v

Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies
check_url/kr/64634?article_type=v

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
check_url/kr/64634?article_type=v

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
check_url/kr/64634?article_type=v

Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis
check_url/kr/64634?article_type=v

Scoring Central Nervous System Inflammation, Demyelination, and Axon Injury in Experimental Autoimmune Encephalomyelitis

Read Article