Journal
/
免疫与感染
/
A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

DOI:
10.3791/64634-v

•

05:44 min

•

December 09, 2022

•

1The Rausing Laboratory, Autoimmunity Section, Division of Neurosurgery, Department of Clinical Sciences,Lund University, 2Department of Autoimmunity,BTB Emulsions

Chapters

  • 00:04Introduction
  • 00:59Material Preparation
  • 01:38Preparation of CFA/Peptide Emulsions
  • 03:31Quality Control of Emulsions
  • 04:01Results: Characterization of Emulsions Produced and EAE Induction in Immunized Mice
  • 05:02Conclusion

Summary

自动翻译

自动翻译

To induce experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis, mice are immunized with a water-in-oil emulsion containing an autoantigen and complete Freund's adjuvant. While several protocols exist for the preparation of these emulsions, a rapid, simple, and standardized homogenization protocol for emulsion preparation is presented here.

Tags

Homogenization MethodAntigen/adjuvant EmulsionExperimental Autoimmune EncephalomyelitisEAECFA SlurryIFA AdjuvantEmulsion PreparationAutoimmune Disease ModelCancer Vaccines

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis

Induction of Experimental Autoimmune Hypophysitis in SJL Mice

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

Author Spotlight: Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis

Author Spotlight: Unveiling the Pathway Linking Obesity to Autoimmune Inflammation in Multiple Sclerosis

Read Article