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使用微泡从人外周血单核细胞样品中分离、活化和扩增基于浮选的 T 细胞
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles
DOI:

06:37 min

December 23, 2022

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Chapters

  • 00:04Introduction
  • 00:29Isolation of T Cells with Microbubbles Using Positive Selection
  • 01:51Co-Stimulation (Activation) of the Positively Selected T Cells
  • 02:52Expansion of the Co-Stimulated Cells in Cell Culture Medium
  • 03:28Expansion of the T Cells (With or Without Prior Transduction)
  • 04:02Harvesting the T Cells and Flow Cytometry
  • 05:16Results: Assessment of Viable and Successfully Transduced Cells
  • 05:58Conclusion

Summary

자동 번역

本研究的目的是证明基于浮选的分离在分离、激活和扩增原代人T细胞方面的可行性。

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