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JoVE Journal
Immunology and Infection

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Evaluation of Caspase Activation to Assess Innate Immune Cell Death
 

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Article DOI: 10.3791/64308-v 10:23 min January 20th, 2023
January 20th, 2023

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요약

This protocol describes a comprehensive method for assessing caspase activation (caspase-1, caspase-3, caspase-7, caspase-8, caspase-9, and caspase-11) in response to both in vitro and in vivo (in mice) models of infection, sterile insults, and cancer to determine the initiation of cell death pathways, such as pyroptosis, apoptosis, necroptosis, and PANoptosis.

Tags

Caspase Activation Innate Immune Cell Death Disease Mechanisms Therapeutic Strategies Endogenous Cells Molecular Mechanisms Bone Marrow BMDMs Influenza A Virus Multiplicity Of Infection Plaque-forming Units PBS High Glucose DMEM Heat Inactivated FBS Incubator
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