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In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
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August 04, 2022
DOI:
1Department of Neurophysiology, Faculty of Medicine,University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine,University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine,The University of Tokyo
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The present protocol describes making a large (6 x 3 mm2) cranial window using food wrap, transparent silicone, and cover glass. This cranial window allows in vivo wide-field and two-photon calcium imaging experiments in the same mouse.
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Manita, S., Shigetomi, E., Bito, H., Koizumi, S., Kitamura, K. In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window. J. Vis. Exp. (186), e64224, doi:10.3791/64224 (2022).
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