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Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
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Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers

Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers

1,833 Views

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11:55 min

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July 12, 2022

DOI:

10.3791/63968-v

DOI:
10.3791/63968-v

•

11:55 min
July 12, 2022

•

5 Views
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1Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School,University of Warwick, 2National Centre for Biological Sciences,Tata Institute of Fundamental Research

Summary

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This protocol describes the formation of supported lipid bilayers and the addition of cytoskeletal filaments and motor proteins to study the dynamics of reconstituted, membrane-tethered cytoskeletal networks using fluorescence microscopy.

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Membrane-tethered Actin CorticesSupported Lipid BilayersCytoskeletal ProteinsReconstitutionLight MicroscopyProtein AssemblyLipid Bilayer FormationUV Curable AdhesiveChamber Fabrication

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Köster, D. V., Bhat, A., Talluri, S., Mayor, S. Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers. J. Vis. Exp. (185), e63968, doi:10.3791/63968 (2022).

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