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Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection
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Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

1,735 Views

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08:02 min

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July 28, 2022

DOI:

10.3791/63830-v

DOI:
10.3791/63830-v

•

08:02 min
July 28, 2022

•

2 Views
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1Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology,Chinese Academy of Sciences, 2University of Chinese Academy of Sciences

Summary

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This methodology, which included oral feeding and intrathoracic injection infection, could effectively assess the influence of midgut and/or salivary gland barriers on arbovirus infection.

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Viral InfectionMosquitoesOral FeedingMicroinjectionAedes AegyptiArbovirusVector CompetenceDengue VirusZika VirusArtificial Feeding SystemAnesthesiaIncubationIntrathoracic InoculationVirus DilutionMicroinjection Needles

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Wang, F., Yang, C., Wang, S., Wu, Q., Ochieng, C., Yuan, Z., Xia, H. Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection. J. Vis. Exp. (185), e63830, doi:10.3791/63830 (2022).

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